Enzyme Activity Experiment
Effect of Temperature on Catalase Activity
Observe how temperature affects the activity of catalase enzyme in breaking down hydrogen peroxide. The amount of foam produced indicates the enzyme activity level.
0°C (Ice Bath)
0°C
25°C (Room Temp)
25°C
37°C (Body Temp)
37°C
25°C
pH 7 (Neutral)
0°C
20°C
40°C
60°C
100%
50%
0%
Time: 0:00
Optimal Temperature
37°C
Enzyme Activity
Medium
Reaction Rate
0.0
Observation:
The experiment will show how temperature affects catalase enzyme activity. More foam indicates more oxygen gas being produced from hydrogen peroxide breakdown.
Enzyme Activity Principles
1. Temperature Effect:
Enzyme activity increases with temperature until the optimal point, then decreases as enzyme denatures.
2. Catalase Reaction:
2H₂O₂ → 2H₂O + O₂ (gas). The oxygen gas produces the foam we observe.
3. Denaturation:
At high temperatures, the enzyme's shape changes permanently, making it non-functional.
