PCR Demonstration
Polymerase Chain Reaction - DNA Amplification Process
Observe how PCR amplifies DNA through repeated cycles of heating and cooling. Watch as the DNA quantity doubles with each cycle!
Temperature: 95°C (Denaturation)
Cycle: 0
Current Stage:
PCR has not started. Click 'Start PCR' to begin the DNA amplification process.
The Science Behind PCR
Key Concepts:
Polymerase Chain Reaction (PCR) is a technique used to amplify specific DNA segments:
- Denaturation: DNA is heated to 95°C to separate strands
- Annealing: Cooled to 50-65°C for primers to bind
- Extension: Heated to 72°C for Taq polymerase to extend primers
- Each cycle doubles the amount of DNA (exponential growth)
Components Needed:
DNA template, primers, Taq polymerase, nucleotides (dNTPs), buffer solution, and a thermal cycler.
