Testing pH of Saliva and Digestive Enzymes – Role of amylase

Saliva pH and Amylase Activity

Saliva pH and Amylase Activity

Testing pH and Digestive Enzyme Function

Investigate how saliva pH affects amylase activity in breaking down starch. Test different conditions and observe the chemical reactions.

Saliva + Starch
pH 7.0
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Current Step:

Select a pH condition and click "Start Experiment" to begin.

Saliva pH and Amylase Activity

Key Concepts:
  • Salivary amylase: Enzyme in saliva that breaks down starch into maltose
  • Optimal pH: Amylase works best at pH 6.7-7.0 (neutral)
  • pH effects: Extreme pH denatures the enzyme, stopping its function
  • Iodine test: Detects starch (blue-black = starch present, yellow = starch digested)
Experimental Steps:
  1. Collect saliva sample
  2. Adjust pH (neutral, acidic, or basic)
  3. Add starch solution
  4. Incubate at body temperature (37°C)
  5. Test for starch with iodine
  6. Observe color change

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